Poietis NGB-R

Scalable automated 4D bioprinting

Next Generation Bioprinting (NGB) plaform has been developed to overcome current tissue manufacturing methods which are the bottleneck impeding standardisation, scale-up and large-scale market adoption of Tissue-Engineering products.

Key Features
- Computer-Assisted Design. NGB platform integrates a specific « cytocentric », user-friendly CAD software.
- Automated, robotic bioprinting with easy-to-use bioprinter based on laser-assisted bioprinting.
- Multimodal traditional extrusion and micro-valve print-head options for complete flexibility in tissue type and complexity.
- In-line monitoring of the print process combined with IA machine learning algorithms
- Tissue formation modeling via dedicated software to program tissue self-organization

Next Generation Bioprinting (NGB) platform has been developed to overcome current tissue manufacturing methods which are the bottleneck impeding standardisation, scale-up and large-scale market adoption of Tissue-Engineering products. It solves critical limitations of existing 3D bioprinting technologies thanks to single-cell resolution and learning-based methods.

Indeed, largely inspired by the principles of the 4.0 Industry, this new platform integrates automation and robotics technologies, coupled with numerous online sensors – including cell microscopy – and Artificial Intelligence processing. In addition, it integrates all bioprinting techniques (laser-assisted bioprinting, bioextrusion, micro-valve bioprinting), a world’s first in the bioprinting market.

Designing 3D Biological Structures with CAD

NGB platform integrates a specific « cytocentric », user-friendly CAD software. This software can be used to design the location and local environment of different cell types and materials in three-dimensional tissue structures.

CAD files describe the architecture of biological tissues with the 3D organisation of tissue components (cells and extracellular matrix).

Integrated Multi-Modal Bioprinting

NGB platform integrates a multimodal, easy-to-use bioprinter based on laser-assisted bioprinting capable of 3D-printing tissue components in sterile conditions, with great accuracy and reliability thanks to 6-axis robotic arm and automation. This bioprinter is now the 4th printer version from the beginning of the project in 2005 and the only fully mutlimodal on the market.

Laser-Assisted Bioprinting in Action

Laser-assisted bioprinting makes it possible to print cell by cell according to the following physical mechanism. The focusing of a laser pulse (in blue) on a cartridge (composed of an ink film spread on a glass plate) results in the formation of an ink jet towards a substrate on which cell microdroplets are collected. By controlling the physical conditions of the ejection (energy, viscosity…), the volume of the droplets is controlled precisely (~ picoliter).
The cell patterns are obtained by rapid scanning of the cartridge by the laser, which results in the formation of 10,000 droplets per second.

Accurately Bioprinting Designs

Bioprinting technologies have been combined with a specific imaging system coupled with AI machine learning algorithms to confirm that what is designed is what is bioprinted.

The Evolution of Bioprinted Constructs

Data generated at all steps of the process are used to model tissue formation. A dedicated software is now under development to program tissue self-organization, which means to anticipate the evolution of the bioprinted construct with time.

Making the Most Out of the NGB-R Bioprinter

All you need to know about the Poietis next generation NGB-R bioprinter:
- 0.22min – 1. Poietis introduction
- 2.35min – 2. NGB-R in a nutshell
- 7.40min – 3. A Cytocentric Approach to Bioprinting
- 9.05min – 3.1. Printing cells separately & no need for commercial bioinks
- 16.22min – 3.2. Defining 4D bioprinting & cell patterning
- 21.00min – 3.3. On-board imaging to take bioprinting farther
Run time: 26.38min